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Cu-SAD data collection by titrating anomalous signal at 17 keV for GHK-GFP crystal form I To examine the phasing limit of the GHK tag, we collected highly redundant Cu-SAD data at a very short wavelength (17 keV, 0.73 Å), where the anomalous scattering from copper is 1.33 e and the calculated Bijvoet ratio for GHK-GFP of 0.62% is significantly smaller and close to the theoretical limit for structure solution (Wang, 1985 ▸)

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Finally, one retrospective study showed good results with azathioprine [34]